Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/18/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Scarborough High School |
| Observations | It is a small black ant. |
| Putative identification | Arthropoda Insecta |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level |
Contamination may have been high because the scientist kept making air bubbles when inserting the samples. There was also a mix up of lanes when inserting the arthropod samples. The scientist also did not add water for the arthropod samples and the water was positive for Wolbachia. So, the trust in my results is low. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Insecta was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica