Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Found by a log on the side of a riverbank. Dark brown/black body, relatively small, legs are a lighter brown with a darker brown color on the joins, furry, small pincers. |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Theridiidae Parasteatoda Parasteatoda tepidariorum |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | There was most likely an error in our conducting of the lab because of our inconclusive results within our gel. This could have been an issue with the pcr or the DNA extraction. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Our results were inconclusive and there were issues with the results of our controls, which made us not confident in our experimental results. Our results are, therefore, invalid. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Baetis_C2.2
Formica_C11
Philaenus sp._C8
Ichneumonidae_A12
Formica_A11