Ant

April 2, 2025

Sample information
Picture
Photos by: Rachel G
Location
Collection date 09/19/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations
  • Banded sugar ant
  • Tail is striped brown and black
  • Ant crawled around tube and curled when it touched the ethanol
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Bar was bright green

Controls worked
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Formicidae was found to be negative for Wolbachia.
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