Carpenter Ant (Hymenoptera)

April 2, 2025

Sample information
Picture
Photos by: Aryaa S (experimented on by Saketha V)
Location
Collection date 02/27/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

The carpenter ant was caught on a rock in front of the school that was near the soil. It was late winter, at around 45 F, with clouds and some wind blowing. It is red and it has 2 long antennae. It has short hair strands coming off of its body. It has 6 legs.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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