Kiki’s Ant

March 31, 2025

Sample information
Picture
Photos by: Andrew S.
Location
Collection date 09/19/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Found this Ant in St. Paul on September 19 of 2024. Temperature was mid 70 roughly 75/76. Arthropod is very small and was found in a ant colony of similar arthropods.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Low beacuse I didn’t have any DNA
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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