Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 09/17/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | It was caught in the fall under a rock. The rock had a wet area and cooler temperature, as well as dirt and mud surrounding it. The bug was black and curled up when it was found. It was caught on September 17th, 2024, in the summer, and it was 73 degrees Fahrenheit. The habitat was urban. |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Woodlouse
Common Water Strider (Aquarius remigis)
Water Strider
Water Strider
Water Skipper