Lady Bug

March 31, 2025

Sample information
Picture
Photos by: Samuel H.
Location
Collection date 09/19/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

It was caught in the fall inside a house; it was on the wall. it was around 4 pm and it was 75°f. It was alone
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA in sixth lane

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Very clearly no band
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Coleoptera was found to be negative for Wolbachia.
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