Roly-poly

March 31, 2025

Sample information
Picture
Photos by: Roman H
Location
Collection date 01/21/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

The bug was found in the dirt under a rock at 9:32 AM. It was early fall and relatively cold outside.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence Download FASTA    Download AB1
GCTCGTGTCnnGAnnnnTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGATAATGCTGGGGA CTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTAC ACACGTGCTACAATGGTGGCTACAACGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGA TTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGG GTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCACTCGAA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TACAGAGTTAGGACAACCTGGTAGGTTAATCGGTGATGACCAGATTTATAATGTAATTGTAACTGCGCATGCTTTCGTGA TAATTTTTTTnAnnnnAATGCCCATTATAATTGGGGGTnnnGGAAATTGACTCnTACCTTTAATGTTAGGAGCTCCTGAT ATAGCTTTCCCTCGTATAAATAATATGAnGTTTTGGTTGTTACCCCCTTCTTTGACTTTACTTCTTAGTAGCGGATTAAT TGAAAGAGGGGTTGGAACCGGGTGAACGGTTTACCCGCCCCTTGCCAGCGGAATCGCTCATAGGGGGGCTTCnnTAnATT TAGGAATTnnnTCGCTTCATTTAnnnGnnnnTTCTTCGATTTTAGGGGCTGTAAATTTTATTACCACTGTAATTAATATG CGAGCTCCGGGGnTTnAAATAnACCGAGTACnnnnnTTTGTTTGGTCTGTCTTGATTACAGCTGTTCTTTTATTACTTTC TCTGCCTGTTTTAGCAnGGGCAATTACAATATTAnTAACCGATCGAAATTnnAnTACTTCTTTTTTTGATCnnnGCGGAG nnnGnnAnCCTATTCTTTAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Armadillidium was found to be postive for Wolbachia.
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