Ant (prenolepis imparis)

March 31, 2025

Sample information
Picture
Photos by: Kieran M.
Location
Collection date 09/20/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

The arthropod was caught in St.Paul during mid-September.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There is no trace of Wolbachia DNA in the electrophoresis gel
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TTGGNNCTTCNATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAAT TCTATAGTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTT AGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCAA TCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGGACAGGGTGAACTGTTTACCCTCCATTAGCTTCTAAT ATTTTTCATAATGGTCCTTCAGTTGACCTAACAATTTTTTCTCTACATATTGCAGGGATATCCTCTATTCTAGGAGCTAT CAATTTTATTTCAACAATTTTAAATATGCGACATAAAAATTTTTCTCCTGATAAAATCCCTTTACTTGTTTGATCTATTT TAATTACAGCAATTTTACTTCTTCTTTCTTTACCAGTTCTAGCAGGAGCTATTACTATACTTTTAACTGACCGAAACCTT AATACTTCTTTTTTTGATCCCTCANGANGGGGGGANCCTGTTCTTTATCAACATTNATTCTGANTTTTTGNTCACC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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