Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/18/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | Small, black-ish brown at with six legs, two long antennae, two pincers, and a large head and thorax. Found on sidewalk in an urban environment in autumn, 77 degrees Fahrenheit. Crawling around on dry leaves, moving slowly on its own, no other ants in sight. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Ant DNA = Lane 2 DNA Ladder = Lane 1 DNA Positive Control = Lane 8 |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Gel electrophoresis did not show Wolbachia presence |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
(no title)
Wolbachia Explored in Hawai’i (Mosquito Negative)
Stripe-eyed flower fly, Eristalinus taeniopa
Lamyctes Emarginatus
Arthropod Collection Lab