Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/19/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Gettysburg College |
| Observations | CO1 sequence:
AAAGATATTGGAACATTATATTTTATTTTTGGGGCTTGAGCAGGAATAGTTGGAACATCCCTTAGTATTTTAATTCGAGCAGAGTTAAGTCAACCTGGAAACTTTATTGGAAATGATCAAATTTATAACGTTATTGTAACAGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTTTAATATTAGGGGCACCAGATATAGCATTTCCTCGAATAAATAATATAAGTTTTTGAATACTACCACCGTCATTAACTTTATTATTATCAAGTAGTTTAGTAGAAAATGGAGCTGGAACTGGATGAACTGTTTATCCTCCCCTTTCTTCTGGAACAGCACATGCAGGAGCTTCCGTTGATTTAGCAATTTTTTCTTTACACTTAGCAGGAATTTCTTCTATTTTAGGGGCCGTAAATTTCATTACTACAGTTATTAATATACGATCATCTGGTATTACTTTGGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTACTATTGTTACTATCTTTACCAGTATTAGCCGGAGCTATTACTATACTTTTAACAGATCGAAATTTAAATACATCTTTCTTTGACCCTATTGGAGGAGGAGATCCTATTTTATACCAACACTTATTCTGATTTTTTGGTNC |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Culex Culex erraticus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Other |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Culex erraticus was found to be negative for Wolbachia. |
(no title)
Big Black Beetle
Fly
Roly Poly Wolbachia in Benu
Citrine Forktail (Ischnura hastata)