Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/19/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Gettysburg College |
| Observations | co1 sequence- ANGATATTGGAACATTATATTTTATTTTTGGGGCTTGAGCAGGAATAGTTGGGACATCTCTGAGTATTTTAATTCGAGCAGAATTAAGTCAACCTGGAAACTTTATTGGAAATGATCAAATTTATAATGTTATTGTAACAGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTTTAATATTAGGAGCACCAGATATAGCATTCCCTCGAATAAATAATATGAGTTTTTGAATACTACCACCGTCATTAACTTTATTATTATCAAGTAGTTTAGTAGAAAATGGGGCTGGAACTGGATGAACTGTTTATCCTCCCCTTTCTTCTGGAACAGCACATGCAGGAGCTTCCGTTGATTTAGCAATTTTTTCTTTGCACTTAGCAGGAATTTCTTCTATTTTAGGGGCCGTAAATTTCATTACTACAGTTATTAATATACGATCATCTGGTATTACTTTAGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTACTATTATTACTATCTTTACCAGTATTAGCCGGAGCTATTACTATACTTTTAACAGATCGAAATTTAAATACATCTTTCTTTGACCCTATTGGAGGAGGAGACCCTATTTTATATCAACACTTATTCTGATTTTT
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| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Psorophora Psorophora ferox |
Methods |
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| Extraction kit | Blood and Tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Process was done 3 times |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
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| Summary | The Psorophora ferox was found to be negative for Wolbachia. |
(no title)
Big Black Beetle
Fly
Roly Poly Wolbachia in Benu
Citrine Forktail (Ischnura hastata)