Ochlerotatus Trivittatus

December 2, 2024

Sample information
Picture
Photos by: Adrianna M., Lizzy C.
Location
Collection date 09/19/2024
Captive / Cultivated? Wild-caught
Group Gettysburg College
Observations

C01- AAGATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGACTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATAGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGGGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTCCCTGTATTAGCCGGAGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAGATCCAATTCTATATCAACATTTATTCTGATTTTTTG
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Aedes Aedes trivittatus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
AAGATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGACTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATAGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGGGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTCCCTGTATTAGCCGGAGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAGATCCAATTCTATATCAACATTTATTCTGATTTTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Aedes trivittatus was found to be negative for Wolbachia.
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