Sample information
Picture	Photos by: Leif R.
Location
Collection date	09/12/2024
Captive / Cultivated?	Wild-caught
Group	St. Paul Academy and Summit School
Observations	it was an ant, it was small and blackish.
Putative identification	Arthropoda Hexapoda Insecta Hymenoptera
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes	Many samples from different arthropods run in gel together
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Hymenoptera was found to be negative for Wolbachia.