April 1, 2025

Sample information
Picture
Photos by: Evie G.
Location
Collection date 09/22/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

I found this ant on a wood fence very close to some soil/woodchips, trees, and flowers. It was sunny and approximately 70 degrees farenheit. I had trouble finding other ants in the area.
Putative identification Arthropoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

samples were ran with positive wolbachia control and negative water control and DNA ladder which goes from 1200bp to 100bp with a bright spot at 700bp. on a piece of parafilm 8 micro liters of DNA and 2 micro liters of loading dye were mixed using a micropipette. The DNA sample was loaded into the gel. The gel was run for 20-30 minutes.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The DNA ladder had many bands, the negative control had no bands, the positive control had multiple bands. My Results showed 2 bands, one at the original site and one a little more towards the negative end of the gel. It tested negative for Wolbachia and positive for DNA.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
NNNNNNNNNNNNNTCTGAGCTGGAATAATTGGATCTTCAATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATAGTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCAATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGGACAGGGTGAACTGTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCTTCAGTTGACCTAACAATTTTTTCTCTACATATTGCAGGGATATCCTCTATTCTAGGAGCTATCAATTTTATTTCAACAATTTTAAATATGCGACATAAAAATTTTTCTCCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACTTCTTCTTTCTTTACCAGTTCTAGCAGGAGCTATTACTATACTTTTAACTGACCGAAACCTTAATACTTCTTTTTTTGATCCCTCAGGAGGGGGGGACCCTGTTCTTTATCAACATTTATTCTGATTTTTTGGTCACCTGGAAANTTAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Formicidae was found to be negative for Wolbachia.
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