Cobweb Spider

April 1, 2025

Sample information
Picture
Photos by: Cormac G
Location
Collection date 10/25/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Caught inside my house, it has 8 legs and a big sack in the rear, it was about 69 degrees inside my house, it was in a corner near a heat source, it was sitting on a cobweb.
Putative identification Arthropoda Chelicerata Arachnida Araneae Theridiidae Steatoda Steatoda triangulosa

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Body without legs
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

There was a clear band on the gel electrophoresis at the exact DNA band length
Wolbachia 16S sequence Download FASTA    Download AB1
TACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCA TGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCC TTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCAC GCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TTCGGGTAGAATTANGACAATCTGGAAGATTGTTAGGTGATGATCAATTATATAATGTAATTGTTACTGG ACACGCTTTTGTAATAATTTTTTTTATAGTAATACCANTATTAATTGGTGGATTTGGAAATTGGTTGGTT CCATTAATATTAGGGGCTCCTGATATAGCTTTTCCTCNAATAAATAATTTAAGATTTTGATTATTACCTC CTTCTTTATTATTATTATTAATTTCTTCTATANAANAAATANGTGTTGGAGCTGGNTGAACTATTTACCC CCCTTTATCTTCTCTGGAAGGTCATANAGGAAGATCTGTANATTTTGCTATNNTNNNTTTACNTTTGGCN GGNNNTTCNTCNATTATAGGTGCTATTAATTTTATTTCTACTATTTTAAATATACGTTTATTTGGGATGA GAATAGAAAAAGTTANACTTTTTGTTTGGTCAGTATTAATCACTGCAGTANTATTATTATTATCNTNACC TGTATTGNCNGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Steatoda triangulosa was found to be postive for Wolbachia.
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