Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 09/18/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | It was warm, and 70 degrees f. The ant was found on the concrete but was surrounded by trees an mulch and a lot of grass. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | High confidence in PCR. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The arthropod band was very strong and there was no Wolbachia band |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
ATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATAGTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCAATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGGACAGGGTGAACTGTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCTTCAGTTGACCTAACAATTTTTTCTCTACATATTGCAGGGATATCCTCTATTCTAGGAGCTATCAATTTTATTTCAACAATTTTAAATATGCGACATAAAAATTTTTCTCCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACTTCTTCTTTCTTTACCAGTTCTAGCAGGAGCTATTACTATACTTTTAACTGACCGAAACCTTAATACTTCTTTTTTTGATCCCTCAGGANGGGGGGANCCTGTTCTTTATCAACATTTATTCTGA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Formicidae was found to be negative for Wolbachia. |
A. punctipennis (Woodland Malaria Mosquito)
Araneae
Woodlouse
Common Water Strider (Aquarius remigis)
Water Strider