Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/20/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | Caught on a very large cottonwood tree next to the soccer field. It was walking very slowly. It was caught in the fall when the temp outside was 70 degrees (F). It has two wings, six legs, and a black body, and it’s covered in small hairs. It also has big eyes. |
| Putative identification | |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | There was no band for Wolbachia, and the controls worked. |
| Wolbachia 16S sequence | Download FASTA
|
| Arthropod COI sequence | Download FASTA
|
| Summary | |
Lamyctes Emarginatus
Arthropod Collection Lab
Dubia Roach
Leaflitter Crab Spider
Arthropod collection (dubia roach)