April 1, 2025

Sample information
Picture
Photos by: Zack B.
Location
Collection date 09/15/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

It was an ant with a three-part body where each section had an equal size. It has six legs and antennae but no wings.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

In the gel image screenshot, my arthropod is lane 5.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My lane has a line where Wolbachia should be, but it’s very faint.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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