Stinkbug

March 9, 2021

Sample information
Picture
Location
Collection date 10/16/2020
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Visible pellet

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Arthropod gel: Controls didn’t work. Water should not have a band.

Wolbachia gel: Wolbachia DNA and controls didn’t work. – control not loaded (mistake made)
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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