Stinkbug

Sample information

Picture
Location
Collection date 10/16/2020
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations
Putative identification Hexapoda Insecta Hemiptera

Methods

Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Visible pellet

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Arthropod gel: Controls didn’t work. Water should not have a band.

Wolbachia gel: Wolbachia DNA and controls didn’t work. – control not loaded (mistake made)

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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