Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/13/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Visible pellet
|
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | Faint Band but Wolbachia DNA didn’t work and water had a faint band Arthropod gel: Arthropod controls didn’t work. Water shouldn’t have a band. Wolbachia gel: Wolbachia DNA didn’t work. Primer dimers found |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Isopoda was found to be postive for Wolbachia. |
Extraction and Analysis of Wolbachia from Halyomorpha halys
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Idaho Beetle
Grammonota