Rollie Pollie

Sample information

Picture
Location
Collection date 10/13/2020
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations
Putative identification Crustacea Malacostraca Isopoda

Methods

Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Visible pellet

 

Results

Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

Faint Band but Wolbachia DNA didn’t work and water had a faint band

Arthropod gel: Arthropod controls didn’t work. Water shouldn’t have a band. 

Wolbachia gel: Wolbachia DNA didn’t work. Primer dimers found

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be postive for Wolbachia.
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