Rollie Pollie

Sample information

Collection date 10/13/2020
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Putative identification Arthropoda Crustacea Malacostraca Isopoda


Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Visible pellet



Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

Faint Band but Wolbachia DNA didn’t work and water had a faint band

Arthropod gel: Arthropod controls didn’t work. Water shouldn’t have a band. 

Wolbachia gel: Wolbachia DNA didn’t work. Primer dimers found

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be postive for Wolbachia.
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