Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by fall 2024 students. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Tephritidae Dioxyna Dioxyna picciola |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | My confidence level was low because the DNA concentration quality values were all below 40, indicating poor quality. The sequencing results were messy, as reflected in the chromatogram, which showed low peaks and bad concentration values. Additionally, when I uploaded the sequence into BLAST, there were no matches found for the DNA sequence in the FASTA format, further reducing my confidence in the data. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Dioxyna picciola was found to be negative for Wolbachia. |
Water Boatman