Sample information |
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| Picture |
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| Location | |||
| Collection date | 09/19/2024 | ||
| Captive / Cultivated? | Wild-caught | ||
| Group | Gettysburg College | ||
| Observations | Lane 3 in gel. Collected from a rural area near a wetland |
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| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Culex Culex erraticus | ||
Methods |
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| Extraction kit | DNeasy (Qiagen) | ||
| DNA extraction location | Abdomen | ||
| Single or Duplex PCR | Single Reaction | ||
| Gel electrophoresis system | Standard electrophoresis system | ||
| Buffer | TBE | ||
| DNA stain | GelGreen | ||
| Gel images |
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| Protocol notes | Lane 3 in gel |
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Results |
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| Wolbachia presence | No | ||
| Confidence level | High | ||
| Explanation of confidence level | Positive positive, negative negative |
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| Wolbachia 16S sequence | |
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| Arthropod COI sequence |
GATATTGGAACATTATATTTTATTTTTGGGGCTTGAGCAGGGATAGTTGGAACATCTCTTAGTATTTTAATTCGAGCAGAATTAAGTCAACCTGGAAACTTTATTGGAAATGATCAAATTTATAATGTTATTGTAACAGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAACTGATTAGTTCCTTTAATATTAGGAGCACCAGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGAATACTACCACCGTCATTAACTTTATTATTATCAAGTAGTTTAGTAGAAAATGGGGCTGGAACTGGATGAACTGTTTATCCTCCCCTTTCTTCTGGAACAGCACATGCAGGAGCTTCCGTTGATTTAGCAATTTTTTCTTTACACTTAGCAGGAATTTCTTCTATTTTAGGGGCCGTAAATTTCATTACTACAGTTATTAATATACGATCATCTGGTATTACTTTAGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTACTATTATTATTATCTTTACCAGTATTAGCCGGAGCTATTACTATACTTTTAACAGATCGAAATTTAAATACATCTTTCTTTGACCCTATTGGAGGAGGAGACCCTATTTTATATCAACACTTATTCTGATTTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Culex erraticus was found to be negative for Wolbachia. | ||
Water Boatman