Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/19/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Gettysburg College |
| Observations | Caught in a rural area, near a wetland |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Aedes Aedes trivittatus |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Lane 4 in gel |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Positive positive, negative negative |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
AAGATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTCCCTGTATTAGCCGGAGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAGACCCAATTCTATATCAACATTTATTCTGATTTTTTG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Aedes trivittatus was found to be negative for Wolbachia. |
Water Boatman