Formica pallidefulva

Sample information
Picture	Photos by: Taken by Jasmine V. of Zahra W.'s insect
Location
Collection date	09/03/2024
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	Collected by Fall 2024 students using an aspirator between the sidewalk by the staff lot near the pond by the staff lot.
Putative identification	Arthropoda Insecta Hymenoptera Formicidae Formica Formica pallidefulva
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	Medium
Explanation of confidence level	Because my Wolbachia BLAST percent identity was 99.72% and not 100%
Wolbachia 16S sequence	Wolbachia pipientis clone A1 16S ribosomal RNA gene, partial sequence BLAST at The Wolbachia Project BLAST at NCBI
Arthropod COI sequence	Formica pallidefulva voucher FKH-010 cytochrome c oxidase subunit I (COX1) gene, partial cds, mitochondrial BLAST at The Wolbachia Project BLAST at NCBI
Summary	The Formica pallidefulva was found to be postive for Wolbachia.

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