Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2024 students using an aspirator between the sidewalk by the staff lot near the pond by the staff lot. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Formica Formica pallidefulva |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | Because my Wolbachia BLAST percent identity was 99.72% and not 100% |
| Wolbachia 16S sequence |
Wolbachia pipientis clone A1 16S ribosomal RNA gene, partial sequence
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
Formica pallidefulva voucher FKH-010 cytochrome c oxidase subunit I (COX1) gene, partial cds, mitochondrial
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Formica pallidefulva was found to be postive for Wolbachia. |
Water Boatman