Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/04/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | It was caught in the grass with a killing jar. |
| Putative identification | Arthropoda Insecta Hymenoptera Vespidae Vespula Vespula maculifrons |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | There was no presence of Wolbachia in my arthropod, but rather it was present in my partner’s. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
>74-C_AG1_Co1R.scf (612 bp) CGAAAAAAGATGTGTTTAAGTTCCGATCTGTTAAAAGTATAGTAATNGCTCCTGCAAGAACTNGTAGTGATAATAGAAGA AGAATTGCTGTAATTAAAATTGATCATGTGAATAAAGGAAGAAAATTTAATGAATGTGTTTTTGTGTGTATATTTAAAAT AGTAACGATAAAATTAATTGATCCTATAATTGATGAAATTCCAGCAATATGAATTGAAAAAATTCCTAAATCTACAGATG GAGAATCATGACCAACAATAGAAGATAAAGGAGGGTATAAAGTTCATCCTGTTCCTACCCCTGTTCCAATAAAATTTCTT AAAATTAGTAAAAATAATGAAGGAGGGAGTAATCAAAATCTTATATTATTTATTCGAGGAAATGCTATATCAGGTACACC TAGTATTAAAGGGATTAATCAGTTTCCAAATCCTCCTACTAAAAAAGGTATAACTATAAAGAAAATTATAATAAAGGCAT GAGCTGTAATAATTGNATTATAAATTTGATCNTTATTAATTAANNNTNCNGGANANCTTAATTCTAAGCNAANAANTATT CTNATNGAAGCTCCTAAAGTCCCTGCCCATNAAGCAAAAANAAAATANAATN
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Vespula maculifrons was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid