Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | collected by fall ’24 students collected using sweep net near bushes
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| Putative identification | Arthropoda Hexapoda Insecta Odonata Libellulidae Erythemis Erythemis simplicicollis |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I do not think I have Wolbachia because I did not have a Wolbachia sequence but i see a faint line on my gel which could be a mixup between me and my partner. |
| Wolbachia 16S sequence |
N/A
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
CTGCTCACGCATTCGTTATAATTTTCTTCATAGTTATGCCTATTATAATTGGTGGCTTTGGAAACTGGTTAGTTCCTTTA ATATTAGGAGCCCCGGATATAGCCTTCCCTCGTCTTAATAATATAAGTTTTTGATTACTTCCTCCCTCCTTCACATTACT TTTATCTAGAAGTATAGTAGAAAGAGGAGCAGGAACAGGATGAACTGTATATCCTCCATTGGCAGGAGCTATTGCACATG CAGGAGCATCTGTAGATTTAACTATTTTCTCGTTACACTTAGCAGGAGTATCCTCCATTCTAGGAGCTATTAATTTTATT ACAACAGTAATCAATATAAAATCCCCTGGGATGAAGCTGGATCAAATACCTCTTTTTGTATGAGCAGTAGTAATTACTGC AGTATTATTACTTTTATCCCTTCCTGTATTAGCAGGAGCTATTACTATACTTTTAACAGATCGTAATATCAACACTTCAT TCTTCGACCCAGCAGG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Erythemis simplicicollis was found to be negative for Wolbachia. |
Water Boatman