Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/04/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2024 students. The moth was caught using a sweep net and a killing jar, on grass near bushes. |
| Putative identification | Arthropoda Insecta Lepidoptera Noctuidae Autographa Autographa precationis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The band for my DNA only showed a positive for arthropod DNA. All controls were correctly shown on the gel. E value for the species was 0.0 and the % identity was 98.57%. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
|
| Summary | The Autographa precationis was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid