Fall 2024

January 7, 2025

Sample information
Picture
Photos by: Josie J
Location
Collection date 09/14/2024
Captive / Cultivated? Wild-caught
Group Frontier High School
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Followed Wolbachia Project Lab Protocol

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

All Controls worked.  All lines were clear and align with DNA ladder
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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