Fall 2024 #2

January 7, 2025

Sample information
Picture
Entry by: Josie J
Location
Collection date 09/14/2024
Captive / Cultivated? Wild-caught
Group Frontier High School
Observations

Collected  outside after it died
Putative identification Arthropoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Followed Wolbachia Lab Protocol for students

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

My ant results matched both positive samples
Wolbachia 16S sequence Download AB1
Arthropod COI sequence Download AB1
Summary The Formicidae was found to be postive for Wolbachia.
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