Fly

January 31, 2025

Sample information
Picture
Photos by: Claire S
Location
Collection date 11/07/2024
Captive / Cultivated? Wild-caught
Group John Bapst Memorial High School
Observations

Black Fly
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain Other
Gel images
Protocol notes

The results for the Black Fly are in the second set of wells titled 1-6.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Since the DNA was not correctly extracted and the lines in the Wolbachia wells were higher than they should have been, levels of confidence for this procedure are low.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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