ANT

February 4, 2025

Sample information
Picture
Entry by: Romi and Shai
Location
Approximate location: 32.15, 34.91
Collection date 12/25/2024
Captive / Cultivated? Wild-caught
Group Katzir High School
Observations

We found an ant, in school yard.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Messor Messor ebeninus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer TBE
DNA stain Other
Gel images
Protocol notes
  1. Caught the ant with our hands
  2. Place it in a Ethanol-filled test tube
  3. DNA extraction process
  4. DNA replication using PCR machine
  5. electrophoresis

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

We saw two bands in our gel. One band of wolcachia and one band of the ant.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Messor ebeninus was found to be postive for Wolbachia.
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