Wolbachia Katzir (Ori Hillel , Liran Shemen)

February 3, 2025

Sample information
Picture
Photos by: Liran Shemen
Location
Collection date 12/25/2024
Captive / Cultivated? Wild-caught
Group Katzir High School
Observations

Caught on a leaf of a big bush.
Putative identification Arthropoda Insecta Hemiptera Lygaeidae Oncopeltus Oncopeltus fasciatus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain Other
Gel images
Protocol notes
  1. Caught the bug with our hands
  2. Place it in a Ethanol-filled test tube
  3. DNA extraction process
  4. DNA replication using PCR machine
  5. electrophoresis

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was only one band that we could see, which was the whole DNA, meaning there is no sight of Wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Oncopeltus fasciatus was found to be negative for Wolbachia.
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