Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/08/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | FernLeaf Community Charter School |
| Observations | it might have been missing a leg |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | We scooped out the abdomen of our spider, following the protocol to the best of our ability. The PCR products we had were not all the way full. The well for the negative control was contaminated with wolbachia, we’re not sure how. Either we switched the labels or accidentally used a pipette tip twice. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | We think we switched our negative control and our positive DNA and some of our PCR products were very minimal. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be negative for Wolbachia. |
(no title)
Arthropod Collection Lab
House Cricket
Week 2/18 lab – Arthropod Collection
Week 2/18 Bio Lab Arthropod Identification