Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/14/2025 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | John Overton High School |
| Observations | The insect was acquired via Bugsincyberspace.com, so we don’t know the exact location of origin. |
| Putative identification | Arthropoda Insecta Coleoptera Carabidae Brachinus Brachinus nigriventris |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Bits of arthropod debris were present during DNA extraction, creating fragments of shells that made smudges on the gel. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | There was contamination present in the samples, as shown by the band in the W- lane. The smudging on the band makes it impossible to detect any bands. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica