Brachinus nigriventris (2)

February 14, 2025

Sample information
Picture
Location
Collection date 02/14/2025
Captive / Cultivated? Captive / Cultivated
Group John Overton High School
Observations

The insect was acquired via Bugsincyberspace.com, so we don’t know the exact location of origin.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Carabidae Brachinus Brachinus nigriventris

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Bits of arthropod debris were present during DNA extraction, creating fragments of shells that made smudges on the gel.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

There was contamination present in the samples, as shown by the band in the W- lane. The smudging on the band makes it impossible to detect any bands.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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