Diptera

March 31, 2021

Sample information
Picture
Location No map location data available.
Collection date 10/11/2020
Captive / Cultivated? Wild-caught
Group William Fremd High School
Observations

Caught in a field with long tall grass. The abdomen of the creature had a long blue fuzz growing on it. Estimated length to be half a centimeter. The pale blue fuzz disappeared when specimen was placed in the collection tube with alcohol. The fuzz has not been identified.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

This was conducted in a lab setting.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The DNA showed a clear test and the controls worked well.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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