Diptera

March 31, 2021

Sample information
Picture
Location No map location data available.
Collection date 10/11/2020
Captive / Cultivated? Wild-caught
Group William Fremd High School
Observations

It was caught in a large field with tall grass.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

This was conducted by professionals in a lab.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The DNA showed a clear test and the controls worked well.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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