Sample information
Picture
Location
Collection date	02/14/2025
Captive / Cultivated?	Captive / Cultivated
Group	John Overton High School
Observations	Acquired through bugsincyberspace.com, so the exact location of origin is unknown.
Putative identification	Arthropoda Hexapoda Insecta Coleoptera Carabidae Brachinus Brachinus nigriventris
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes
Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary