Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Captive / Cultivated |
| Group | John Overton High School |
| Observations | Acquired online, so exact location of origin is unknown. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Carabidae Brachinus Brachinus nigriventris |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Arthropod debris was present during DNA purification, leading to shell fragment in the sample and smudges on the gel. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Smudges were present at the end of the gel and no bands were present. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Monochamus Scutellatus MMH10
Eleodes Armata MMH9
Gryllus Veletis MMH8
Trochosa Ruricola MMH7
Apis Mellifera MMH6 L6