Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/14/2025 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | John Overton High School |
| Observations | Specimen acquired through bugsincyberspace.com, so the exact location of origin is unknown. |
| Putative identification | Arthropoda Hexapoda Insecta Blattodea Blaberidae Gyna Gyna centurio |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Smudges are present on the gel, likely caused from arthropod debris in the sample. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | There is some evidence of contamination on the gels, but this is the only sample that showed bands. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Gyna centurio was found to be postive for Wolbachia. |
Ladybug
Armadillidium vulgare #1 GRP30
Drosophila Melanogaster
Armadillidium vulgare GRP7 (1)