Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/14/2025 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | John Overton High School |
| Observations | Specimen acquired through bugsincyberspace.com, so the exact location of origin is unknown. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Gyrinidae Gyrinus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Arthropod debris was present in the sample after DNA purification, leading to arthropod fragments and smudges in the gel. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Smudges were present on the gel. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Baetis21Cb2.1
Diptera21Aa2.1
Baetis21Aa6
Baetis21Aa2.3
Diptera_Cb6