Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/12/2025 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Berkshire Community College |
| Observations | This arthropod was collected in Petco(a local pet store & captive colony). |
| Putative identification | Arthropoda Hexapoda Insecta Blattodea Blaberidae Blaptica Blaptica dubia |
Methods |
|
| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | |
| Buffer | |
| DNA stain | |
| Gel images |
|
| Protocol notes | For DNA extraction we used the insect adaptation of the protocol. Taq Polymerase used: New England Biolabs OneTaq. Annealing temperature for PCR reaction: 49 C. |
Results |
|
| Wolbachia presence | |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Protocol 1
Arthropod 2
Arthropod 1 nosy pill woodloosen
Arthropod 1 (Millipede)
Arthropod 2 – Roach