Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 02/12/2025 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Berkshire Community College |
| Observations | This arthropod was collected in Petco(a local pet store & captive colony). |
| Putative identification | Arthropoda Hexapoda Insecta Blattodea Blaberidae Blaptica Blaptica dubia |
Methods |
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| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | For DNA extraction we used the insect adaptation of the protocol. March 4th, 2025: Single PCR with Arthropod primers. Taq Polymerase used: New England Biolabs OneTaq. Annealing temperature for PCR reaction: 49 C. March 25th, 2025: Duplex PCR with Arthropod and Wolbachia primers. Taq polymerase used: NEB OneTaq Hot Start Quick-Load 2x MM w/ Stand Buffer (M0244S). Annealing temperature for PCR reaction: 49 C.
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Results |
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| Wolbachia presence | |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Black Blow Fly
arthropod 2
Rachel S. Fruit Fly
Mealworm Beetle
T. Viridis (Long-Jawed Orbweaver)