Captive Blaptica Dubia Cockroach

February 18, 2025

Sample information
Picture
Photos by: Emily O.
Location
Collection date 02/12/2025
Captive / Cultivated? Captive / Cultivated
Group Berkshire Community College
Observations

This arthropod was collected in Petco(a local pet store & captive colony).
Putative identification Arthropoda Insecta Blattodea Blaberidae Blaptica Blaptica dubia

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

For DNA extraction we used the insect adaptation of the protocol.

March 4th, 2025:

Single PCR with Arthropod primers.

Taq Polymerase used: New England Biolabs OneTaq.

Annealing temperature for PCR reaction: 49 C.

March 25th, 2025:

Duplex PCR with Arthropod and Wolbachia primers.

Taq polymerase used: NEB OneTaq Hot Start Quick-Load 2x MM w/ Stand Buffer (M0244S).

Annealing temperature for PCR reaction: 49 C.

April 11, 2025:

Running gel electrophoresis once again to look at results of March 25th’s Duplex PCR.

Taq Polymerase used: New England Biolabs OneTaq Hot Start Quick-Load 2x MM w/ Standard Buffer (product # M0488S).

DNA Ladder used: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S).

 

 

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

I am not confident with my results because there are no clear bands in our samples (MRH6 and ELMO8). There were smears, which indicates that the DNA concentration will need to be reevaluated.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
Report Inappropriate Post