February 18, 2025

Sample information
Picture
Photos by: Harold P.
Location
Collection date 03/29/2025
Captive / Cultivated? Captive / Cultivated
Group Berkshire Community College
Observations

It is Juvenile, it has 6 legs, 2 antennas. It was collected in a captive colony, the borders of it are dark. Abdomen has some dark spots.
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Taq Polymerase used: New England Biolabs OneTaq Hot Start Quick-Load 2x MM 2/ Standard Buffer (product # M0488S)

DNA Ladder used: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S)

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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