Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/29/2025 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Berkshire Community College |
| Observations | It is Juvenile, it has 6 legs, 2 antennas. It was collected in a captive colony, the borders of it are dark. Abdomen has some dark spots. |
| Putative identification | Arthropoda Insecta Blattodea Blaberidae Blaptica Blaptica dubia |
Methods |
|
| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Taq Polymerase used: New England Biolabs OneTaq Hot Start Quick-Load 2x MM 2/ Standard Buffer (product # M0488S) DNA Ladder used: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S)
|
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid