Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/18/2025 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Berkshire Community College |
| Observations |
|
| Putative identification | Arthropoda Insecta Coleoptera Tenebrionidae Tenebrio Tenebrio molitor |
Methods |
|
| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Taq polymerase used: New England Biolabs One Taq Hot Start Quick-Load 2x MM w/ Standard Buffer (product # M0488S) DNA Ladder used: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # N0559S) Single PCR W/ Arthropod Primers 3/4/25 49c Duplex PCR W/ Wolbachia Primers & Arthropod Primers 3/25/25 49c |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | In the first Gel, there was a smear present suggesting too much DNA. The second Gel had nothing present. It’s inconclusive because I couldn’t seem to find the right amount of DNA to test. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid