Sample information |
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Picture |
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Location | |
Collection date | 02/17/2025 |
Captive / Cultivated? | Wild-caught |
Group | Berkshire Community College |
Observations | -Upon collection Arthropod appeared |
Putative identification | Arthropoda Crustacea Malacostraca Decapoda |
Methods |
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Extraction kit | Monarch DNA extraction (NEB) |
DNA extraction location | Partial abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Edvotek Gel Electrophoresis |
Buffer | TAE |
DNA stain | SYBR Safe |
Gel images |
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Protocol notes |
Single Arthropod PCR set up on March 6th, 2025 to amplify fragment of the CO1 cytochrome oxidase gene using Arthropod_F (LCO1490) and Arthropod_R (HCO2198) Primers with an annealing temperature of 49°C. Separate Single Wolbachia PCR set up on March 27th, 2025 to amplify fragment of the 16S ribosomal RNA gene using Wolbachia_F (16S_WspecF) and Wolbachia_R (16S_WspecR) Primers with an annealing temperature of 55°C. |
Results |
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Wolbachia presence | No |
Confidence level | Medium |
Explanation of confidence level | |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Decapoda was found to be negative for Wolbachia. |