February 21, 2025

Sample information
Picture
Photos by: Sierra W
Location
Collection date 02/04/2025
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations

Six ants (A1-A6) and one mosquito (M1) were collected and assessed. Presence of Wolbachia was observed in all six ants while no presence of WalB was indicated in mosquito collected. All controls worked accordingly.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Ochetellus Ochetellus glaber

Methods
Extraction kit pcr
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Six ants scraped off the side of home in Aina loa, Hawai’i at 7:30am on a sunny morning. M1 mosquito was collected on 1/31/25 at 6:30 am inside home of Aina loa, Hawai’i and was 68 degrees outside. PCR preparation and standard Gel electrophoresis prepared and ran. Two PCR tests examined including Co1 and WalB primers.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Six ants showed positive results for Wolbachia, although some bands are clearer represented than others Wolbachia definitely was observed.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Ochetellus glaber was found to be postive for Wolbachia.
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