Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/02/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State Lehigh Valley |
| Observations | Found in the landscape |
| Putative identification | Arthropoda Insecta Coleoptera Carabidae Harpalus Harpalus pensylvanicus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system Bio-Rad |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | |
| Wolbachia 16S sequence |
No Priming
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
TTTTTTNNNNNGNNTGAGCNNGAATAGTAGGAACTTCATTAAGTATATTAATTCGAACTGAATTGGGGACTCCTGGTGCA TTGATTGGTAATGATCAAATTTATAATGTTATTGTTACTGCTCATGCTTTCGTAATAATTTTTTTTATAGTTATACCTAT TATAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGCCTTTCCTCGAATAAATAACA TAAGTTTTTGATTACTTCCTCCTTCTTTAACTCTTCTCTTAATGAGAAGTATAGTGGAAAGAGGAGCTGGTACTGGATGA ACAGTTTACCCACCTTTATCATCTAGAATTGCTCATAGAGGAGCTTCAGTAGATTTAGCAATTTTTAGATTACATTTAGC TGGAATTTCATCAATTTTAGGTGCAGTAAACTTTATTACTACCATTATTAATATGCGATCAGTAGGAATAACATTTGATC GAATACCATTATTTGTATGATCAGTAGGAATTACAGCATTACTTTTACTTTTATCATTACCAGTATTAGCAGGAGCTATT ACTATATTACTTACAGATCGAAATTTAAATACTTCATTTTTTGATCCTGCCGGAGGAGGAGATCCTATTTTATACCAACA TTTATTTTGATTTTTTGGTCACCCNNGAAAGTTTAA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Harpalus pensylvanicus was found to be postive for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica