Brown Stink Bug – Arthropod 1

March 5, 2025

Sample information
Picture
Photos by: Jayant K. Jamie K
Location
Collection date 12/01/2024
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Caught in the classroom

 
Putative identification Arthropoda Insecta Hemiptera Pentatomidae Halyomorpha Halyomorpha halys

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

The PCR protocal was optimized according to barcoding gene and wolbachia. The 1st group was heated for optmized results for the barcoding gene and 2nd group was heated for optmized results for wolbachia.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

We are novices
Wolbachia 16S sequence
Arthropod COI sequence
NNNNNTNNCTTCGGATATGATCTGGAATAGTAGGATCAGCTATAAGATTAATTATCCGTATCGAATTAGGACAGCCTGGA AGATTTATTGGTAATGATCAAATTTATAATGTAATTGTAACAGCACATGCATTTGTAATAATTTTCTTTATAGTAATACC AATCATAATTGGAGGATTCGGTAATTGATTAGTCCCTTTAATAATTGGAGCACCTGATATAGCCTTCCCACGATTAAATA ATATAAGATTCTGATTATTACCCCCTTCATTAACTTTATTATTAATAAGAAGAATAGCAGAATCAGGAGCTGGGACAGGA TGAACAGTCTATCCCCCCTTATCAAGTAATATCTCACATAGAGGAGCATCAGTTGATTTAGCTATTTTTTCCTTACATTT GGCTGGGGTTTCATCAATTTTAGGGGCAGTAAATTTTATTTCAACTATTATAAATATACGACCAACAGGTATAACCCCTG AACGAATCCCATTGTTTGTGTGATCAGTAGGAATTACTGCACTTCTTCTGTTATTATCCTTACCTGTGTTAGCAGGTGCT ATTACTATACTATTAACTGACCGAAATTTTAATACCTCTTTTTTTGACCCATCAGGAGGAGGAGACCCTATTCTTTATCA ACATTTATTTTGATTTTTTGGTCACCCNGGAAGTTNAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Halyomorpha halys was found to be negative for Wolbachia.
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