Sample information |
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Picture |
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Location | |
Collection date | 12/01/2024 |
Captive / Cultivated? | Wild-caught |
Group | Penn State Lehigh Valley |
Observations | Caught in the classroom
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Putative identification | Arthropoda Hexapoda Insecta Hemiptera Pentatomidae Halyomorpha Halyomorpha halys |
Methods |
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Extraction kit | DNeasy (Qiagen) blood and tissue kit |
DNA extraction location | Abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system Bio-Rad |
Buffer | TAE |
DNA stain | SYBR Safe |
Gel images |
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Protocol notes | The PCR protocal was optimized according to barcoding gene and wolbachia. The 1st group was heated for optmized results for the barcoding gene and 2nd group was heated for optmized results for wolbachia. |
Results |
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Wolbachia presence | No |
Confidence level | Medium |
Explanation of confidence level | We are novices |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Halyomorpha halys was found to be negative for Wolbachia. |