Trichotichnus fulgens 2B2

April 2, 2025

Sample information
Picture
Photos by: Daniel D
Location
Collection date 02/27/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

Collected on the ground, under a rock, next to stream. It is a tiny black beetle, it has 6 legs and 2 antennae coming from its head. Its shell is a shiny black and its legs are slightly yellow/orange.
Putative identification Arthropoda Insecta Coleoptera Carabidae Stenolophus Stenolophus ochropezus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

clearly no band
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
>2B2 AGTAGGGACTTCCTTAAGTATACTAATTCGAGCTGAATTAGGAACTCCCGGTGCATTAATTGGTGATGATCAAATTTATA ATGTTATTGTAACTGCACATGCTTTTATCATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGA TTGGTACCTTTAATATTAGGAGCTCCTGATATAGCCTTTCCTCGAATAAATAATATAAGTTTTTGATTATTGCCTCCTTC ATTAACTCTTCTCCTAATAAGAAGAATAGTAGAAAGAGGAGCTGGGACTGGATGAACAGTTTACCCACCCCTATCATCTG GTATTGCCCATGGAGGTGCTTCAGTTGACTTAGCTATTTTTAGTCTACATTTAGCAGGAGTATCTTCTATTCTAGGAGCT GTAAATTTTATTACTACAATTATTAATATACGATCAGTAGGAATAACATTTGATCGAATACCATTATTTGTTTGATCTGT AGGAATTACTGCTCTACTTCTACTTTTATCTTTACCTGTATTAGCAGGAGCAATTACTATATTGTTAACAGATCGAAATC TAAATACTTCATTTTTTGACCCAGCGGGAGGGGGAGATCCTATTTTATATCAACATTTATTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Stenolophus ochropezus was found to be negative for Wolbachia.
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