Black Blow Fly – Arthropod 2

April 1, 2025

Sample information
Picture
Photos by: Cora O
Location
Collection date 02/25/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Caught in Penn State Lehigh Valley bathroom.
Putative identification Arthropoda Insecta Diptera Calliphoridae Phormia

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

We are novices.
Wolbachia 16S sequence
Arthropod COI sequence
TNNNNNNTTCGGAGCTTGATCTGGAATAATTGGAACTTCATTAAGAATTCTAATTCGAGCTGAACTAGGGCACCCTGGAG CTCTAATTGGAGATGACCAAATTTATAACGTAATTGTAACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCA ATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGGGCTCCTGATATAGCTTTCCCACGAATAAACAA TATAAGTTTTTGACTTTTACCTCCTGCATTAACTCTATTGTTAGTTAGTAGTATAGTAGAAAATGGGGCTGGAACAGGAT GAACTGTTTACCCACCTTTATCATCTAATATTGCTCATGGAGGAGCATCTGTTGATCTAGCTATTTTCTCTCTTCACTTA GCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTCATTACAACTGTAATTAATATACGATCAACTGGAATTACATTTGA TCGAATACCTTTATTTGTTTGATCTGTAGTTATTACTGCTCTATTACTTTTATTATCTTTACCTGTATTAGCCGGTGCTA TTACTATATTATTAACTGATCGAAATTTAAACACTTCATTCTTTGACCCAGCAGGAGGAGGAGATCCTATTTTATATCAA CACTTATTCTGATTTTTTGGTCACCCNNGAAGTTTAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Phormia was found to be negative for Wolbachia.
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