Sample information
Picture	Photos by: Cora O
Location
Collection date	02/25/2025
Captive / Cultivated?	Wild-caught
Group	Penn State Lehigh Valley
Observations	Caught in Penn State Lehigh Valley bathroom.
Putative identification	Arthropoda Hexapoda Insecta Diptera Calliphoridae Phormia
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system Bio-Rad
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level	We are novices.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Phormia was found to be negative for Wolbachia.